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Dr. F. Went noted that if coleoptile tips were removed and placed on agar for one hour, the agar would produce a bending when placed on one side of freshly-cut coleoptile stumps. Of what significance is this experiment?

1. It made possible the isolation and exact identification of auxin.
2. It is the basis for quantitative determination of small amounts of growth-promoting substances.
3. It supports the hypothesis that IAA is auxin.
4. It demonstrated polar movement of auxins.

Correct answer: It made possible the isolation and exact identification of auxin.

Solution

The experiment showed that the coleoptile tip released a diffusible growth-promoting substance into agar, and that this substance could still cause bending when applied to a stump. That made it possible to collect the active compound and eventually isolate and identify auxin.

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