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For its action, nitrogenase requires

1. Mn²⁺
2. light
3. high input of energy
4. super oxygen radicals

Correct answer: high input of energy

Solution

Nitrogenase catalyzes the reduction of N₂ to NH₃, a reaction that is energetically expensive because nitrogen gas is very stable. The enzyme therefore requires a high input of energy, typically supplied through ATP and reducing power.

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