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The stage during which separation of the paired homologous chromosomes begins is

1. Pachytene
2. Diplotene
3. Zygotene
4. Diakinesis

Correct answer: Diplotene

Solution

In diplotene, homologous chromosomes begin to separate after crossing over, but they stay attached at chiasmata. This is the stage where the paired chromosomes start moving apart while still visibly connected.

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