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Which of the following test cannot be used for identifying amino acids ?

1. Ninhydrin test
2. Barfoed Test
3. Xanthoproteic test
4. Biuret test

Correct answer: Barfoed Test

Solution

Ninhydrin, xanthoproteic and biuret tests are all used for amino acids/proteins. The Barfoed test distinguishes monosaccharides from reducing disaccharides (a carbohydrate test) and cannot be used to identify amino acids.

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