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Which reagent is used to identify the N-terminal amino acid residue of a peptide?

1. p-Toluenesulphonyl chloride
2. 2,4-Dinitrophenylhydrazine
3. Carboxypeptidase
4. 2,4-Dinitrofluorobenzene

Correct answer: 2,4-Dinitrofluorobenzene

Solution

The N-terminal residue is identified with Sanger's reagent, 2,4-dinitrofluorobenzene (DNFB), which reacts with the free alpha-amino group to give a yellow DNP-amino acid that survives hydrolysis and can be identified.

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