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An amino acid has a carboxyl group with pKₐ1 = 2.34 and an ammonium group with pKₐ2 = 9.60. At what pH will this amino acid have its isoelectric point?

1. 5.97
2. 2.34
3. 9.60
4. 6.97

Correct answer: 5.97

Solution

For a neutral amino acid the isoelectric point is the mean of the two relevant pKa values: pI = (2.34 + 9.60)/2 = 5.97.

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